Spot indole test: evaluation of four reagents.

Evaluation of media and reagents for indole-spot tests in anaerobic bacteriology.

Differentiation of Haemophilus spp. in Respiratory isolate cultures by an indole spot test.

Indole spot tests using isolated, nonhemolytic colonies of Haemophilus species were positive for 90 of 151 (60%) respiratory isolates of Haemophilus influenzae, whereas 67 to 72 (93%) isolates of H. influenzae from cerebrospinal fluid and blood specimens were indole positive. Only 4 of 117 (3%) Haemophilus parainfluenzae isolates were positive for indole spot tests. Thus, indole-positive, nonhe...

Comparison of three reagents for detecting indole production by anaerobic bacteria in microtest systems.

Three reagents for detecting indole, Kovac, Ehrlich, and p-dimethylaminocinnamaldehyde (DMCA), were evaluated with commercial microtest systems for characterizing and identifying anaerobic bacteria. The DMCA reagent, the most sensitive of the three reagents, gave a positive reaction with 445 of 449 strains of various indole-producing anaerobic bacteria. There was 99.6% agreement between the res...

Development and evaluation of a miniaturised method as an aid to the identification of clinically important anaerobic bacteria.

A miniaturised method for the identification of anaerobic bacteria is described which employs microtitre fermentation tests, a spot indole test and a nitrate reduction disc test. The results obtained are directly comparable with those produced by a standard conventional method in use at present.

Identification of Indole-3-Acetic Acid in the Basidiomycete Schizophyllum commune.

Indole-3-acetic acid (IAA) was detected in the ether extracts of culture filtrates of indigotin-producing strains of the basidiomycete Schizophyllum commune. Several solvents, known to give distinctly different R(F) values for IAA, and 3 location reagents gave identical results with synthetic IAA and IAA found in the extract. Confirmation was obtained by the Avena straight growth test, split pe...